The Spittelbrunnen is a famine well, i.e. a karst spring that periodically carries water, in the north of the Hainich near Mühlhausen in Thuringia.

The Spittelbrunnen, which only pours after long periods of rain, is located in the Mühlhausen city forest and is lined with shell limestone blocks. The spring has a diameter of around one and a half meters and is about two meters deep. The well takes its name from the former village of Spudelborn, which was abandoned in the 13th century. The outflowing Spittelgraben runs to the east and merges into the Felchtaer Bach, a tributary of the Unstrut, at Weidensee.